The Single-Cell World Podcast

Ep.46: Piccolo- A New Normalization Method for scRNA-seq

Join us on this podcast episode as I explore the fascinating world of single-cell RNA sequencing (scRNA-seq) data normalization with Amartya Singh, a post-doctoral researcher at Reuters. Discover the limitations of the standard methods used in scRNA-seq data normalization and how Amartya's innovative solution is changing the game. Hear firsthand about his journey of inspiration and the ideas that led to the development of this groundbreaking approach. Don't miss out on this insightful discussion - tune in now!
Useful Links:
Amartya Singh LinkedIn and GitHub profile
The starting point publication 
TuBA paper
Piccolo paper
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Ep 45: High Resolution Pathology: Molecular Cartography from Resolve Biosciences

In this insightful podcast interview with Kieren Patel, the Chief Business Officer at Resolve Biosciences, we delve into the world of Molecular Cartography technology. Kieren provides details about this innovative platform, underscoring its remarkable capabilities in facilitating high molecular resolution pathology. Additionally, Kieren sheds light on forthcoming solutions set to be commercialized later this year, with a particular emphasis on their commitment to multi-omics and customized approaches. Tune in to gain valuable insights into the latest advancements in molecular cartography and discover the promising future that Resolve Biosciences' offerings hold.
 
Useful links:
Resolve Biosciences
website
LinkedIn
Twitter
Youtube
Datasets
Kieren Patel
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Ep. 44: Scale Biosciences Innovating scDNA methylation and scRNAseq

In this podcast episode, I had the privilege of talking with Melanie Masuda, the Director of Market Development at Scale Biosciences. Melanie told me all about Scale Biosciences' cutting-edge technology and gave me a full overview of all of their different kits.
 
Listen now and understand how the single-cell (sc) sequencing DNA methylation, the scRNAseq, and the CRISPR enrichment kits work. Maybe this is the solution for your research project!
 
Useful Links:
Melanie Masuda LinkedIn
Scale Biosciences(website)
Twitter
LinkedIn
KITS
scDNA methylation
scRNA
CRISPR guide enrichment kit
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Ep.43: The SMART-seq Family-full length RNA methods

In this insightful podcast episode, I have the privilege of interviewing Simone Picelli, the Head of the Single-Cell Genomics Platform at the Institute of Ophthalmology Basel. As we talked, we got into the specifics of single-cell RNA sequencing (scRNA-seq) full-length methods from the SMART-seq family.
Simone shared valuable insights into various methods within, including SMART-seq, SMART-seq 2, SMART-seq 3, FLASH-seq, FLASH-seq low-amplification, and FLASH-seq with UMI. We explored the unique features and applications of each method, highlighting their contributions to advancing single-cell genomics research. 
In addition, we also talked about other common methods and the bigger picture of single-cell sequencing technology, such as the problems it faces and its future.
Explore the fascinating world of SMART-seq methods with Simone Picelli - a must-listen episode. Tune in now and learn about the ideal way to study RNA isoforms. 

Ep. 42: Updating Episode 14, Single-Cell Methods-an overview

The Single-Cell World Podcast is now available in video as well, but just on Apple Podcast and in our new YouTube channel  😉
Episode 14: Single-Cell Methods, an overview, needed an update since there is a fourth method that is being used to individualize cells when using single-cell sequencing technology.
Join me and listen to it, as well as other updates concerning the methods that are at the core of the single-cell sequencing technology.
Here I leave you a gift, related with this episode.Thank you for listening.Kind Regards,Cátia

Ep.41: Single-Cell Sequencing Products 2023 Review_Part 2

Hi there!
Today is Part 2 of the review of single-cell-related products launched in 2023. I hope I don't miss any. If I do, send me a message telling me about it. 😉
Here are some useful links:
Exploring Single-Cell Sequencing online course
Companies that I talk during this episode (part 2):
Scale Biosciences
Scipio Bioscience
Singleron Biotechnologies
Takara Bio
10X Genomics
Free Resources
Sponsorship
Pay me a Ko-Fi
Thanks for listening!
Cheers,
Cátia

Ep. 40: Single-Cell Sequencing Products Review 2023_Part1

Hi there!
Today I review single-cell-related products launched in 2023. They are a lot! I hope I don't miss any. If I do, send me a message telling me about it. 😉
Here are some useful links:
Join the Single-Cell World Newsletter
Companies that I talk during this episode (part 1):
BD Biosciences 
Ep. 18: Samples Multiplexing
BioScryb Genomics
Fluent BioSciences
Episode 31: Podcast Fluent BioSciences
Honeycomb Biotechnologies
Episode 19: Podcast Honeycomb Biotechnologies
Mission Bio
Episode 16: Podcast Mission Bio
Parse Biosciences 
Episode 35: Podcast Parse Biosciences
Free Resources
Sponsorship
Pay me a Ko-Fi
Thanks for listening!
Cheers,
Cátia

Ep. 39: My Contribution to Frontline Genomics Spatial & Single-Cell Analysis Playbook

Hello there!
The Spatial and Single-Cell Analysis Playbook was released last week. Front Line Genomics produced it. I was honored to be invited to take part in it.
In this episode, you can hear the questions that were asked (AI voice here) and my responses during this fantastic interview.
I hope you like it!
Useful Links
Free Resources
The Single-Cell World website
Sponsorship
Pay me a Ko-Fi
Thanks for listening.Cheers,Cátia