Ep.43: The SMART-seq Family-full length RNA methods

In this insightful podcast episode, I have the privilege of interviewing Simone Picelli, the Head of the Single-Cell Genomics Platform at the Institute of Ophthalmology Basel. As we talked, we got into the specifics of single-cell RNA sequencing (scRNA-seq) full-length methods from the SMART-seq family.

Simone shared valuable insights into various methods within, including SMART-seq, SMART-seq 2, SMART-seq 3, FLASH-seq, FLASH-seq low-amplification, and FLASH-seq with UMI. We explored the unique features and applications of each method, highlighting their contributions to advancing single-cell genomics research. 

In addition, we also talked about other common methods and the bigger picture of single-cell sequencing technology, such as the problems it faces and its future.

Explore the fascinating world of SMART-seq methods with Simone Picelli - a must-listen episode. Tune in now and learn about the ideal way to study RNA isoforms. 

USEFUL LINKS:

• Simone Picelli Information
• LinkedIn
• Laboratory

Publications

• SMART-Seq
• SMART-Seq 2 Original Paper
• SMART-Seq 2 Method Paper
• SMART-seq 3
• SMART-Seq 3 Express
• FLASH-seq

Protocols:

• FLASH-Seq
• FLASH-Seq UMI
• FLASH-Seq low amplification
• Bulk FLASH-Seq

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