The Single-Cell World Podcast

Ep.14: Single-Cell Methods: an overview

Welcome!In this episode I will talk about the 3 main methods that were the base for the different single-cell sequencing technology. Below you can find all companies and products links that are mentioned during this episode. I hope this is useful for your and somehow can help. Thanks for listening!PLATE-BASED METHODS (e.g. commercial options)96 well Plates (BioRad)REPLI-g Advanced DNA Single Cell Kit (Qiagen)SMART-Seq Single Cell Kit (Takara Bio)Evercode Whole Trancriptome (Parse Biosciences)Micro, Nano or Pico WELL-BASED METHODS (e.g. commercial options)HIVE scRNAseq (Honeycomb Biotecnologies)GEXScope Single Cell RNA kits (Singleron)ICELL8 system (Takara Bio)DROPLET-BASED METHODS (e.g. commercial options)Tapestri (Mission Bio)Chromium Controller (10X Genomics)Nadia (Dolomite Bio)DNBelab C Series (MGI)USEFUL LINKSSingle-Cell Course: Exploring the Cutting-Edge World of Single-Cell Technology (May 9, 2023-July 18, 2023Pay me a Ko-fi

Ep. 13: Working With Nuclei

Hi there!In this episode I talk with Dr. Luciano Martelotto, an expert on single-nuclei experiments. When we cannot isolate happy cells for our experiments, nuclei are the next option that we have. Listen now to learn more about this topic.Who is Dr. Luciano Martelotto?He is A/Professor at the Head of the Development Laboratory of the Adelaide Centre for Epigenetics (ACE), South Australia ImmunoGENomics Cancer Institute (SAIGENCI). Prior to joining ACE/SAIGENCI, he was the Scientific Director of the Single Cell Laboratory at Harvard Medical School, Department of Systems Biology, Harvard University (USA). During his role at the Single Cell Lab at HMS he led a team of technology specialists in charge of developing, implementing, and offering methods of single-cell profiling to build a bridge between researchers and single-cell technologies in their quest to understand how cells collectively perform systems-level functions in healthy and diseased states.He is also a Technology Advisor for multiple companies including Omniscope, Paragon Genomics, Honeycomb Bio, ArgeTAG, Truckee Applied Genomics and South Australian Genomics Centre.Luciano has a robust interdisciplinary scientific background, specialising in molecular biology and biochemistry with a strong background in technology and engineering. He is originally a cell and molecular biologist with a degree in Biotechnology, a PhD in Biological Sciences and Post-doctoral training in cancer genomics and single cell sequencing technologies. His diverse scientific background stems from working in a wide range of fields – plants genetics, microbiology, cancer biology and genomics – and has shaped my ambition and capacity to learn, create, develop, and apply technologies that cross multiple disciplines. The common thread woven throughout his career path has been the focus on converging ideas from various sciences and taking this ideology to develop or refine approaches to address challenges in real life settings and ultimately contribute to improving lives through technological advancements.email: luciano.martelotto@adelaide.edu.auTwitter: @LGMartelottoLinkedIn: Luciano G. MartelottoUsefull linksSaltyEZ10/50 protocolDaughter of Frankenstein protocolFrankenstein ProtocolMULTI-seq: Sigma Product & ProtocolJob opportunity 1Single-Cell Course: Exploring the Cutting-Edge World of Single-Cell Technology (May 9, 2023-July 18, 2023Pay me a Ko-fi

Ep. 12:Dead cells. Now what?

In this podcast episode, I talk about different strategies that we can use when we have a lot of dead cells in our samples. Below there are links for the products and machines I mentioned on this episode.SINGLE-CELL COURSE, May 9, 2023Support this podcastDEAD CELL REMOVAL KITSMiltenyi BiotecAkadeumStem CellREAGENTSFicoll-PaqueLymphoprepHistopaqueOptiprepMACHINESLuna FLLeviCell

Ep.11: Sample Preservation Methods For Single-Cell Experiments

Hello there!Season two of The Single-Cell Podcast begins today.In this episode, I discuss the various preservation methods that can be used on samples for single-cell sequencing experiments.TopicsCryopreservationSnap-freezingFixationSpecific single-cell strategies developed by various single-cell sequencing technologies like Singleron, 10X Genomics, Honeycomb Biotechnologies, Parse BiosciencesThe courseExploring the Cutting-Edge World of Single-Cell Technology: An Introductory CourseFor more detailsPay me a kofi to support this podcastThank you!

Ep. 10: SINGLE-CELL CONSULTATIONS: Question & Answers

To celebrate that The Single-Cell World podcast has already 10 episodes, I decided to do something different. In this episode I share with you some frequent questions that I get on my talks and scientific consultations.Besides that, tell you that this is the last episode of season 1. I will be back in 2 weeks after my holidays.In the meanwhile, luck for your single-cell experiments!

Ep. 09: Automated Tissue Dissociators For Single-Cell Experiments

What about if I tell you that you don't have to do your tissue dissociations manually?In this episode I talk about different automated machines options that we can use to get single cells but also single nuclei. Next Thursday blog post you will find more information about this. BTW, I don't have any agreement or professional/personal link with single-cell or biotec companies. My recomendations are based on colleagues/my experiences.Gentle Mechanical Tissue Dissociators:gentleMACS Dissociator (Miltenyi)Singleron Python Tissue Dissociator (Singleron)Tissue Grinder (Fast Foward Discoveries)DSC-400 Single Cell Suspension Dissociator (RWD Life Science)VIA Extractor (Cytiva)Singulator (S2 Genomics)Medimachine (BD Biosciences)Thanks for listening!

Ep. 08: Solid Samples: phases of sample preparation

Working with solid samples?In this episode I will talk about the different phases of solid samples preparation.In next Thursday blog post you will find more information concerning solid samples. However I leave you here links from some of the websites and products that I mention during the podacast.BTW, I don't have any agreement or professional/personal link with single-cell or biotec companies. My recomendations are based on colleagues/my experiences.WEBSITES:Protocols.ioPubmedResearch GatebioRxivCELL COUNTERSLuna FLCytosmartThanks for listening!

Ep. 07: Liquid Samples: what was used in single-cell sequencing?

Working with liquid samples?In this episode I will tell you what samples were used on the published single-cell sequencing works. I will explain also the most common sample preparation methods used for these type of samples.In next Thursday blog post you will find more information concerning liquid samples. However I leave you here links from some of the products that I mention during the podacast.BTW, I don't have any agreement or professional/personal link with single-cell or biotec companies. My recomendations are based on colleagues/my experiences.PRODUCTS:Strainers: FlowmiTM Cell Strainers, MACS SmartStrainers, and pluriStrainersGradient Denity Solutions: Ficoll-Paque, Lymphoprep, Histopaque, and OptiprepMagnetic Columns: MiltenyiThanks for listening!