The Single-Cell World Podcast

Ep. 22: Talking About Single-Cell Data Analysis (Part 2)

Hello there,Today's episode is Part two of “Talking about single-cell data analysis”, with Ayoub Lasri, founder of Single-Cell Analytics. In this episode, Ayoub explains better what type of services Single-Cell Analytics offers and how they can help us in our projects.Listen now!Useful Links:Single-Cell Analytics LTD WebsiteLinkedInTwitterAyoub Lasri contact: ayoub-lasri@singlecell-analytics.comSc-RNA ToolsSupport this podcastThanks for listening!Cátia

Ep. 21: Talking About Single-Cell Data Analysis (PART 1)

Hello there,In today's episode Ayoub Lasri, co-founder of Single-Cell Analytics, outlines the data processing method for single-cell RNA seq data in this episode. This podcast was split into two parts because there is so much helpful information to give. This is part one!Join us and see how much fun we have while discussing single-cell data analysis. I'm sure you're curious about whether my assumptions about single-cell data analysis as a wet-lab researcher are correct or incorrect.Useful Links:Single-Cell Analytics LTD WebsiteLinkedInAyoub Lasri contact: ayoub-lasri@singlecell-analytics.comSupport this podcastThanks for listening!Cátia

Ep. 20. The Singulator: revolutionising tissue dissociation

Hi there,Join me for an exciting episode as I talk with John Bashkin, Chief Strategy Officer of S2 Genomics, about the Singulator - an automated tissue dissociator designed specifically for single-cell experiments.Discover how S2 Genomics is revolutionizing tissue dissociation at the single-cell level and how the Singulator enables us to get clean suspensions of cells or nuclei for our experiments. John provides fascinating insights into the Singulator's many applications, ranging from cancer research to organoids research.Whether you're interested in exploring different approaches on tissue dissociation or simply curious about the latest developments on it, this episode is not to be missed! Tune in to learn more about Singulator technology and the future of sample preparation.Useful LinkS2 Genomics: Website , Twitter, LinkedInJohn Bashkin, contact: johnb@s2genomics.comSupport this podcastThanks and see you in other episode.Cátia

Ep. 19. The HIVE CLX Solution: Any Cell. Any Where. Any Time

Hi there,In this episode I have a special guest, Irene Whitney the senior director of commercial strategy from Honeycomb Biotechnologies. We talk about their unique single-cell technology, the HIVE single-cell solution that allows us to study any cell, anywhere, and anytime.She also updates me about the new versions of the HIVE system, the HIVE CLX, explaining how it works and most important, the benefits of using this technology in single-cell research compared to traditional single-cell methods. Join me for this exciting episode as I explore the cutting-edge world of single-cell technology with Irene Whitney from Honeycomb Biotechnologies.Useful Links:Honeycomb Biotechnology: Website , Twitter, LinkedInIrene Whitney, contact: irene@honeycomb.bioHIVE CLXHIVE resourcesBeeNet PlusCellometer AutoSUPPORT THIS PODCAST

Ep. 18: Pooling or Multiplexing Samples?

Hi there,In this podcast episode, I will tell you the difference between pooling or multiplexing samples for a single-cell experiment. I will also explain the type of tags that we can use to multiplex samples: genotype, antibodies or lipids. Finaly I will let know about the benefits of using this strategies, like single-cell cost reduction. Here are the links for the products I mention at this episode:Genotyping (SNP) Arrays Examples: Illumina ; ThermoFisherBiolegend Antibodies & single-cell experimentsLipid Tags: Multi-seq (Sigma)👉🏻 Support this Podcast!Listen now!Cheers,CátiaDon't forget to subscribe to our newsletter and blog.

Ep.17 Types of single-cell mRNA sequencing assays

Hi there,In this podcast episode, I will tell you what three of single-cell mRNA assays we have available in order to study gene expression.👉🏻 Whoul you like to support this Podcast?Listen now!Cheers,CátiaDon't forget to subscribe to our newsletter and blog.

Ep.16: Mission Bio: single-cell targeted DNA & more

Hi!In this exciting episode, I talk with Gema Fuerte, Mission Bio's Field Application Scientist Manager for EMEA. Gema takes us on an exciting journey through the world of single-cell genomics and the revolutionary technologies developed by Mission Bio to study individual cells in unprecedented detail.We discuss Mission Bio's goal of revolutionizing disease research at the single-cell level, as well as how their cutting-edge Tapestri platform and kits enable the detection of rare cell populations and genetic mutations that contribute to disease.Gema provides fascinating insights into Mission Bio's technology's many applications, ranging from cancer research to translational research and clinical trials in biopharma companies and academia. This episode is not to be missed if you are a scientist interested in exploring the frontiers of single-cell DNA analysis or simply curious about the latest developments in the life sciences. Join us as we explore Mission Bio's exciting world and the future of single-cell genomics.MISSION BIO WebsiteTwitterLinkedInGema Fuerte, contact: fuerte@missionbio.comSUPPORT THIS PODCASTPay me a Ko-fi

Ep15: Single-Cell DNA Sequencing Methods: an overview

Hi there,In this podcast episode, I will be talking about single-cell DNA. DNA is the information necessary to build our bodies. Alterations in DNA can cause changes in cell behavior, leading to diseases.To study DNA at the single-cell level, we can use diferent protocols. Some examples are the whole-genome or the targeted approaches, the scATAC-seq or the scDNA methulation protocols. Several commercial solutions are available and I will also mention them.Listen now!Cheers,CátiaUsefull LinksLAST DAY!! Single-Cell Course: Exploring the Cutting-Edge World of Single-Cell Technology (May 9, 2023-July 18, 2023Support this PodcastDNA ALTERATIONS KITS:REPLI-g Advanced DNA Single Cell Kit (Qiagen)GenomePlex® Single Cell Whole Genome Amplification Kit (Merck)illustra™ Single Cell GenomiPhi™ DNA Amplification Kit (Merck)Pico PLEX® Single Cell WGA Kit (Takara Bio)AMPLI1 WGA PLUS KIT (Silicon biosystems)MALBAC Single Cell WGA Kit (Yikon genomics)ResolveDNA (Bioscryb Genomics)scDNAseq kit (Mission Bio)CHROMATIN ACESSIBILITY KITS:ICELL8 scATAC-seq (Takara Bio)scATAC-seq (10X Genomics)SureCell ATAC-Seq Library Prep Kit (Biorad)DNA METHYLATION KITS:xGen™ Methylation-Sequencing DNA Library Preparation Kit (Integrated DNA Technologies)TWIST NGS Methylation Detection System (Twist Bioscience)scTAMseq(Mission Bio)