Ep15: Single-Cell DNA Sequencing Methods: an overview

Hi there,

In this podcast episode, I will be talking about single-cell DNA. DNA is the information necessary to build our bodies. Alterations in DNA can cause changes in cell behavior, leading to diseases.

To study DNA at the single-cell level, we can use diferent protocols. Some examples are the whole-genome or the targeted approaches, the scATAC-seq or the scDNA methulation protocols.

Several commercial solutions are available and I will also mention them.

Listen now!
Cheers,
Cátia

Usefull Links

• LAST DAY!! Single-Cell Course: Exploring the Cutting-Edge World of Single-Cell Technology (May 9, 2023-July 18, 2023
• Support this Podcast

DNA ALTERATIONS KITS:

• REPLI-g Advanced DNA Single Cell Kit (Qiagen)
• GenomePlex® Single Cell Whole Genome Amplification Kit (Merck)
• illustra™ Single Cell GenomiPhi™ DNA Amplification Kit (Merck)
• Pico PLEX® Single Cell WGA Kit (Takara Bio)
• AMPLI1 WGA PLUS KIT (Silicon biosystems)
• MALBAC Single Cell WGA Kit (Yikon genomics)
• ResolveDNA (Bioscryb Genomics)
• scDNAseq kit (Mission Bio)

CHROMATIN ACESSIBILITY KITS:

• ICELL8 scATAC-seq (Takara Bio)
• scATAC-seq (10X Genomics)
• SureCell ATAC-Seq Library Prep Kit (Biorad)

DNA METHYLATION KITS:

• xGen™ Methylation-Sequencing DNA Library Preparation Kit (Integrated DNA Technologies)
• TWIST NGS Methylation Detection System (Twist Bioscience)
• scTAMseq(Mission Bio)